免疫缺陷 RetS812F/+ 小鼠的局部肠道神经缺陷与神经介导的肛门直肠收缩力降低

Materials availability

B6.Cg-Sirpa < Non-obese diabetic (NOD)> Rag2 < tm1Fwa> Il2rg<tm1Sug>/Jic (B6RGS) mice in this study are available from the Central Institute for Experimental Medicine and Life Science (CIEM). To generate B6RGS-Ret^S812F/+ mice, frozen sperm from male C57BL/6N-Ret^S812F/+ mice should be obtained from Kobe University. The parent 1231A3 human-induced pluripotent stem cell lines should be provided from Yamanaka laboratory. Requests for further resources should be directed to and will be fulfilled by the lead contact, Makoto Ikeya (ikeya-g@cira.kyoto-u.ac.jp).

Acknowledgements

We sincerely thank the former and current members of the T-CiRA Ikeya project, especially Yayoi Toyooka, Daisuke Kamiya, Teppei Akaboshi, and Hideyuki Hiyoshi, for their valuable support; Mika Sato and Yoshiki Yamaguchi from Orizuru Therapeutics for their technical assistance; and Yoshiaki Kassai, Yasushi Kajii, and Shinya Yamanaka for critically evaluating this study.

Author information

Authors and Affiliations

1. T-CiRA Discovery and Innovation, Takeda Pharmaceutical Company Limited, Fujisawa, Kanagawa, Japan

   Yusaku Komoike, Teruyoshi Yamashita, Taiki Nakajima & Hirokazu Matsumoto

2. Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan

   Yusaku Komoike, Teruyoshi Yamashita, Taiki Nakajima, Hirokazu Matsumoto & Makoto Ikeya

3. Central Institute for Experimental Medicine and Life Science (CIEM), Kawasaki, Kanagawa, Japan

   Motohito Goto & Masafumi Yamamoto

4. Division of Neural Differentiation and Regeneration, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan

   Mukhamad Sunardi & Hideki Enomoto

5. Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan

   Makoto Ikeya

Authors

1. Yusaku Komoike
2. Teruyoshi Yamashita
3. Taiki Nakajima
4. Motohito Goto
5. Masafumi Yamamoto
6. Mukhamad Sunardi
7. Hirokazu Matsumoto
8. Hideki Enomoto
9. Makoto Ikeya

Corresponding author

Correspondence to Makoto Ikeya.

Ethics declarations

Competing interests

Yusaku Komoike, Teruyoshi Yamashita, Taiki Nakajima and Hirokazu Matsumoto are employees of Takeda Pharmaceutical Company Limited. Motohito Goto and Masafumi Yamamoto are employees of Central Institute for Experimental Medicine and Life Science. Financial support was provided by Takeda Pharmaceutical Co Ltd. The other authors declare no competing interests.

Grant support

This study was supported by a T-CiRA budget from Takeda Pharmaceutical Company, Limited.

Disclosures

All authors had access to the study data and reviewed and approved the final manuscript.

Lead contact

Requests for further information should be directed to the lead contact, Makoto Ikeya (ikeya-g@cira.kyoto-u.ac.jp).

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Cite this article

Komoike, Y., Yamashita, T., Nakajima, T. et al. Localized enteric neural deficiency and reduced nerve-mediated anorectal contractility in immunodeficient Ret^S812F/+ mice. Sci Rep (2026). https://doi.org/10.1038/s41598-026-75879-1

Download citation

• Received: 07 August 2026

• Accepted: 09 October 2026

• Published: 16 October 2026

• DOI: https://doi.org/10.1038/s41598-026-75879-1

Keywords